Publications
Trusted and validated by global research communities.
- A high-throughput droplet digital PCR system aiming eight DNA methylation targets for age prediction
- Development and evaluation of a triplex droplet digital PCR method for differentiation of M. tuberculosis, M. bovis and BCG
- Development of an Integrated Disposable Device for SARSCoV-2 Nucleic Acid Extraction and Detection
- Digital Counting of Breaks Labeling In Situ A Fast and Absolute Quantification Method for Measurement of DNA Double-Strand Breaks Based on Digital Polymerase Chain Reaction
- Digital Melting Curve Analysis for Multiplex Quantification of Nucleic Acids on Droplet Digital PCR
- Establishment and Application of Multiplex Droplet Digital PCR for SARS-CoV-2 Omicron Variant
- Ｈ5 亚型高致病性禽流感病毒微滴式数字 PCR 检测方法的建立与应用
- Ｈ7 亚型高致病性禽流感病毒微滴式数字 PCR 检测方法的建立与应用
- Mismatch-Guided Deoxyribonucleic Acid Assembly Enables Ultrasensitive and Multiplex Detection of Low-Allele-Fraction Variants in Clinical Samples
- Multiplex Digital PCR-Based Development and discussion of the Detection of Genetic Association Between Staphylococcus aureus and mecA
- 基因 VI 型新城疫病毒反转录数字 PCR 检测方法的建立与应用
- 基因 Ⅶ.2 亚型新城疫病毒反转录数字 PCR 检测方法的建立与应用
- 小反刍兽疫病毒液滴式数字 PCR 检测方法的建立及应用
- 尼帕病毒 N 基因逆转录微滴式数字 PCR 检测方法建立
- 微滴式数字 PCR 定量检测伯氏考克斯氏体方法的建立及应用
- 新型冠状病毒 B.1.1.7 变异株特异性数字 PCR 定量方法研究
- A Stable Cell Line-Based Genomic DNA Reference Material for Accurate Hpv 45 Detection
- Development of RT‑dPCR method and reference material for rotavirus G3P8 and G9P8
- Establishment of Digital PCR Method and Reference Material for Adenoviruses 40 and 41
- Establishment of digital PCR method and reference materials for porcine parvovirus detection
- Establishment of genomic RNA reference materials for BCR‑ABL1 P210 measurement
- Establishment of potential reference measurement procedure and reference materials for EML 4-ALK fusion variants measurement
- Establishment of pseudovirus reference material for human norovirus GII.4
- Establishment of reference measurement procedure and reference material for Treponema pallidum
- IHHNV假病毒核酸标准物质定值研究
- Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR
- Reference material development for detection of human respiratory syncytial virus using digital PCR
- 内含赤羽病病毒 S 基因假病毒的构建、鉴定及初步应用
- 牛结节性皮肤病病毒（NMG 株）基因组 DNA 标准物质的研制
- 霍乱弧菌核酸检测试剂国家参考品的建立
- 非洲马瘟病毒（AV1311 株）核糖核酸标准物质的研制
- 靶向药物甲磺酸伊马替尼的分子标志物 BCR-ABL 融合基因定量检测平台的质量评价方法的研究
- A New Multiplex Genetic Detection Assay Method for the Rapid Semi-Quantitative Detection of Six Common Curable Sexually Transmitted Pathogens From
- CircNF1 modulates the progression and immune evasion of esophageal squamous cell carcinoma through dual regulation of PD-L1
- COVID-19 mitigates the response to TKIs in patients with CML via the inhibition of T-cell immunity
- COVID-19 Rebound After VV116 vs Nirmatrelvir-Ritonavir Treatment A Randomized Clinical Trial
- Developing a multiplex PCR-based assay kit for bloodstream infection by analyzing genomic big data
- Development of a Multiplex ddPCR Detection Method for Accurate Non-Invasive Prenatal Testing of Trisomy 21
- Digital PCR-based GRHL2 methylation testing in acute myeloid leukemia diagnosis prognosis and monitoring
- Mapping Longitudinal Lung Mycobiome Characteristics of Severe COVID-19 patients, a prospective, multicenter cohort study
- Usefulness of KIT mutant transcript levels for monitoring measurable residual disease
- 数字 PCR 与实时荧光定量 PCR 检测慢性髓性白血病患者 BCR::ABL mRNA 水平的比较性研究
- 高灵敏微滴式数字 PCR 检测低水平 JAK2 V617F 突变
- A Novel AAV Capsid-Mediated RS1 Gene Therapy Restored Retinal Function to Wild-Type Levels in Rs1R213W Mouse Model
- Development and validation of optimized lentivirus-like particles for gene editing tool delivery with Gag-Only strategy
- Electrostatic microfiltration (EM) enriches and recovers viable microorganisms at low-abundance in large-volume samples and enhances downstream detection
- PlexinA1 (PLXNA1) as a novel scaffold protein for the engineering of extracellular vesicles
- Rapid Universal Detection of High-Risk and Low-Abundance Microbial Contaminations in CAR-T Cell Therapy
- A PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration
- A Strategy for the Production and Molecular Validation of Agrobacerium-Mediated Intragenic Octoploid Strawberry
- Analysis of microbial diversity and volatile metabolites across different types of pit mud in sauce-flavored Baijiu based on nanopore sequencing and metabolomics
- Development of Droplet Digital PCR Assay for Detection of Seed-Borne Burkholderia glumae and B. gladioli Causing Bacterial Panicle Blight Disease of Rice
- Doped carbon dots affect heavy metal speciation in mining soil: changes of dissimilated iron reduction processes and microbial communities
- Establishment of a Sensitive and Reliable Droplet Digital PCR Assay for the Detection of Bursaphelenchus xylophilus
- Identification and functional verification of Y-chromosome-specific gene typo-gyf in Bactrocera dorsalis
- Inhibitory Effect and Mechanism of Trichoderma taxi and Its Metabolite on Trichophyton mentagrophyte
- Nanoparticle LDH enhances RNAi efficiency of dsRNA in piercing-sucking pests by promoting dsRNA stability and transport in plants
- QuantAS: a comprehensive pipeline to study alternative splicing by absolute quantification of splice isoforms
- Systemically Silencing Long Non-coding RNAs Maclpil With Short Interfering RNA Nanoparticles Alleviates Experimental Ischem
- The circular RNA circANK suppresses rice resistance to bacterial blight by inhibiting microRNA398b-mediated defense
- 利用实时荧光定量PCR和数字PCR检测鉴定水稻细菌性条斑病菌
Newest Posts
Microchemical Journal
2025 Jun
• The RT-dPCR method was established to accurately quantify the pseudovirus RM for norovirus GⅡ.4.
• The pseudovirus RM for norovirus GⅡ.4 comprises ORF2 RNA and demonstrates homogeneity and stability.
• The pseudovirus RM with an assigned value can serve as a quality control tool for the detection methodology targeting norovirus GⅡ.4.
Jiayi Yang, Mingwei Liu, Yunhua Gao, Huijie Li, Lianhua Dong
Invest Ophthalmol Vis Sci
2025 Jun 2
A novel mouse model harboring the patient-derived RS1 missense variant R213W was generated, accurately recapitulating the human phenotype. We developed a novel AAV serotype, AAV.IVT18, which efficiently transfected photoreceptor and bipolar cells by intravitreal injection, and optimized the expression cassettes of RS1.
Xing Wei, Sisi Ma, Yunyu Zhou, Haoliang Cui, Shan Zhang, Duanyang Wang, Yingying Fu, Wei Li, Huihui Han, Yamei Li, Wuyi Li, Huixin Liu, Zixi Sun, Xiaoxu Han, Xuan Zou, Hui Li, Cheng Wang, Ruifang Sui
Microchemical Journal
2025 May
• HPV 45 RM development using C-33A cell line with full-length genome integration.
• High-accuracy dPCR method with LoD 8.57 and LoQ 16.8 copies/reaction for RM characterization.
• Certified RM facilitates global standardization of HPV DNA detection.
Shujun Zhou, Hongbo Hu, Yuping Li, Wenjun Dai, Yi Yang, Jun Lin, Lianhua Dong
Eur J Med Res
2025 Apr 4
Keywords: Base editing; Gag-only; Lentivirus-like particles (LVLPs); MRNA; Packaging signal.
Jinlin Jia, Yanzhe Hao Lu Zhang, Xiaofang Cao, Lisha An, Hu Wang, Qi Ma, Xiaohua Jin, Xu Ma
Plant Cell
2025 Apr 2
The lack of effective tools to specifically knock down circRNAs without affecting host gene expression has slowed the progress of plant circRNA research. Here, we have developed a CRISPR-Cas13d tool that can specifically knock down circRNAs in plant systems, successfully achieving the targeted knockdown of circRNAs in rice (Oryza sativa).
Xiaohui Liu, Peihong Wang, Sai Wang, Weixue Liao, Mingyan Ouyang, Sisi Lin, Rongpeng Lin, Panagiotis F Sarris, Vasiliki Michalopoulou, Xurui Feng, Zinan Zhang, Zhengyin Xu, Gongyou Chen, Bo Zhu
Small Methods
2025 Mar 30
Keywords: cell therapy sterility test; digital LAMP; electrostatic microfiltration; multiplex nucleic acid testing; quantitative detection of pathogens.
Xiaolin Wu, Joshua Jebaraj Raymond, Yaoping Liu, Alexander Jeremias Odermatt, Wei-Xiang Sin, Denise Bei Lin Teo, Meenubharathi Natarajan, Inn Chuan Ng, Michael E Birnbaum, Timothy K Lu, Jongyoon Han, Stacy L Springs, Hanry Yu
Cell Mol Biol Lett
2025 Mar 29
Keywords: ANXA1; Esophageal squamous cell carcinoma (ESCC); PD-L1; STAT3; circRNAs.
Chang Wang, Chenxi Ju, Dan Du, Peiyu Zhu, Jie Yin, Jinlin Jia, Xue Wang, Xinyu Xu, Li Zhao, Junhu Wan, Ting Sun, Lijun Yang, Hongle Li, Fucheng He, Mingxia Zhou, Jing He
Research Article
2025 Mar
Keywords: lung; Mycobiome; COVID-19; ddPCR.
Xiaoqing Dai, Meng Cao, Zunliang Wang
Biosensors (Basel)
2025 Jan 10
Keywords: digital melting curve analysis; droplet digital PCR; fluorescence imaging; molecular diagnostics; multiplex detection.