{"id":8994,"date":"2025-05-23T11:10:26","date_gmt":"2025-05-23T03:10:26","guid":{"rendered":"http:\/\/vibrojet-tech.com\/?post_type=docs&#038;p=8994"},"modified":"2026-05-26T13:57:02","modified_gmt":"2026-05-26T05:57:02","password":"","slug":"quantas-a-comprehensive-pipeline-to-study-alternative-splicing-by-absolute-quantification","status":"publish","type":"docs","link":"https:\/\/vibrojet-tech.com\/?docs=quantas-a-comprehensive-pipeline-to-study-alternative-splicing-by-absolute-quantification","title":{"rendered":"QuantAS: a comprehensive pipeline to study alternative splicing by absolute quantification of splice isoforms"},"content":{"rendered":"\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-f9a80f8f wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-1ba7d75e wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<p class=\"has-text-color has-link-color wp-elements-02ae535c6d2e2e64f8e0e2fd1e7181b8 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:600\">New Phytol.\u00a0<\/p>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-e8eed1186eadd779c275b88884d95fdd wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#070a12;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\">2023 Nov<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-1ba7d75e wp-block-group-is-layout-flex\" style=\"padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<p class=\"has-text-color has-link-color wp-elements-d1d1198cf0e2020df5aac118aaf58633 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#070a12;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\">DOI:<\/p>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-e006952ba8bb54f20cd1fb947cf637b2 wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300\">10.1111\/nph.19193<\/p>\n<\/div>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-3cbaf9173385f40858dd18c29233823f wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#029ae6;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0;font-size:14px;font-style:normal;font-weight:300;line-height:1.4\">Yu-Chen Song<sup>\u00a01\u00a02<\/sup>,\u00a0Mo-Xian Chen<sup>\u00a01\u00a02<\/sup>,\u00a0Kai-Lu Zhang<sup>\u00a01\u00a02<\/sup>,\u00a0Anireddy S N Reddy<sup>\u00a03<\/sup>,\u00a0Fu-Liang Cao<sup>\u00a01<\/sup>,\u00a0Fu-Yuan Zhu<sup>\u00a01\u00a02<\/sup><\/p>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-wide\" style=\"margin-top:0;margin-bottom:0;background-color:#eaeef0;color:#eaeef0\"\/>\n\n\n\n<h2 class=\"wp-block-heading has-text-color has-link-color wp-elements-f5d8ab3aab5e4f03af7ffeedf44e63d6\" style=\"color:#070a12;margin-top:var(--wp--preset--spacing--70);margin-bottom:var(--wp--preset--spacing--50);font-size:24px\">Abstract<\/h2>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-83aa24131476a1318820d3ef0d8dc1af wp-block-paragraph\" style=\"color:#5c6066;margin-top:0px;margin-right:0;margin-bottom:12px;font-size:14px;line-height:1.4\">In general, QuantAS provides an accurate quantitative method for isoform quantification, reduces systematic experimental errors, and exhibits high compatibility and specificity. By contrast, other methods such as HiFENS, which is based on fluorescence in situ hybridization (FISH) for the detection of endogenous splicing isoforms, still depend on isoform-specific regions (Shilo et al., [21]).<\/p>\n\n\n\n<p class=\"has-text-color has-link-color wp-elements-5fff65664886a40dc931f1ba4380aef6 wp-block-paragraph\" style=\"color:#5c6066;margin-bottom:var(--wp--preset--spacing--20);font-size:14px;line-height:1.4\">The method adopts isoform-specific primers to solve the difficulty in isoform identification caused by various alternative splicing (AS) events. It takes functional coding regions as the classification unit of isoform structures to ensure the independence of each isoform (Fig.).<\/p>\n\n\n\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-340403b9 wp-block-group-is-layout-flex\" style=\"margin-top:0;margin-bottom:0;padding-top:var(--wp--preset--spacing--50);padding-right:0;padding-bottom:var(--wp--preset--spacing--50);padding-left:0\">\n<h2 class=\"wp-block-heading has-text-color has-link-color wp-elements-b7e294eb63435ee1fe036bb97c83c849\" style=\"color:#5c6066;font-size:14px\"><strong>Tag<\/strong>s:<\/h2>\n\n\n\n<p class=\"has-text-color has-background has-link-color wp-elements-7fa9413f320b1eb24445f2618daa624f wp-block-paragraph\" style=\"border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#9fa7ad;background-color:#f5f6f7;padding-top:12px;padding-right:16px;padding-bottom:12px;padding-left:16px;font-size:14px;font-style:normal;font-weight:600\">absolute quantification; alternative splicing; digital PCR; QuantAS; quantitative real-time PCR<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Develops the QuantAS pipeline for absolute quantification of alternative splicing, extending the value of absolute quantification concepts into plant transcriptomics.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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