{"id":8961,"date":"2025-05-23T09:59:18","date_gmt":"2025-05-23T01:59:18","guid":{"rendered":"http:\/\/vibrojet-tech.com\/?post_type=docs&p=8961"},"modified":"2026-05-25T17:38:46","modified_gmt":"2026-05-25T09:38:46","password":"","slug":"establishment-of-digital-pcr-method-and-reference-materials-for-porcine-parvovirus-detection","status":"publish","type":"docs","link":"https:\/\/vibrojet-tech.com\/?docs=establishment-of-digital-pcr-method-and-reference-materials-for-porcine-parvovirus-detection","title":{"rendered":"Establishment of digital PCR method and reference materials for porcine parvovirus detection"},"content":{"rendered":"\n
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Microchemical Journal.\u00a0<\/p>\n\n\n\n

2024 Dec<\/p>\n<\/div>\n\n\n\n

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DOI:<\/p>\n\n\n\n

10.1016\/j.microc.2024.112143<\/p>\n<\/div>\n\n\n\n

Caihang\u00a0Zhang,\u00a0Yanmin\u00a0Lin,\u00a0Jiayi\u00a0Yang,\u00a0Yu\u00a0Zhang,\u00a0Xia\u00a0Wang,\u00a0Lianhua\u00a0Dong,\u00a0Jingya\u00a0Yang<\/p>\n\n\n\n

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Highlights<\/h2>\n\n\n\n

\u2022 Digital PCR technology is absolute quantification and does not rely on standard curves for quantification.<\/p>\n\n\n\n

\u2022 A reference method and reference material for detecting porcine parvovirus were established.<\/p>\n\n\n\n

\u2022 Validation was performed on different digital PCR platforms.<\/p>\n<\/div>\n\n\n\n

Abstract<\/h2>\n\n\n\n

Porcine parvovirus (PPV) is a common virus, which can cause reproductive disorders of sows and great losses to pig farms. In addition, it is also crucial to conduct testing for PPV in biologics derived from porcine sources. In this study, we had established a reference method based on digital PCR (dPCR) technology for Porcine Parvovirus detection, developed reference material (RM), and studied the related characteristics. The established PPV dPCR method showed good linearity within five orders of magnitude (R\u00b2 > 0.999). The reference value and extended uncertainty of PPV RM was (2.60 \u00b1 0.23) \u00d7 10\u2076 copies\/\u03bcL. This study demonstrates that the establishment of dPCR method and RM enable accurate quantification of the PPV gene, improve accuracy in PPV detection, and help improve diagnostic methods to better guide clinical treatment.<\/p>\n\n\n\n

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Tag<\/strong>s:<\/h2>\n\n\n\n

Porcine Parvovirus (PPV); Digital PCR (dPCR); Reference Material (RM); Reference value and uncertainty<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Establishes a digital PCR method and reference materials for porcine parvovirus, supporting quantitative standardization and quality control of animal viral nucleic acid testing.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"footnotes":""},"doc_category":[31],"doc_tag":[],"class_list":["post-8961","docs","type-docs","status-publish","hentry","doc_category-standardization-reference-materials"],"year_month":"2026-05","word_count":201,"total_views":0,"reactions":{"happy":0,"normal":0,"sad":0},"author_info":{"name":"root","author_nicename":"root","author_url":"https:\/\/vibrojet-tech.com\/?author=1"},"doc_category_info":[{"term_name":"Standardization & Reference Materials","term_url":"https:\/\/vibrojet-tech.com\/?doc_category=standardization-reference-materials"}],"doc_tag_info":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/8961","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs"}],"about":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/types\/docs"}],"author":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8961"}],"version-history":[{"count":6,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/8961\/revisions"}],"predecessor-version":[{"id":14822,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/8961\/revisions\/14822"}],"wp:attachment":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8961"}],"wp:term":[{"taxonomy":"doc_category","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_category&post=8961"},{"taxonomy":"doc_tag","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_tag&post=8961"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}