{"id":6614,"date":"2024-12-24T10:05:54","date_gmt":"2024-12-24T02:05:54","guid":{"rendered":"http:\/\/vibrojet-tech.com\/?post_type=docs&p=6614"},"modified":"2026-05-26T11:38:50","modified_gmt":"2026-05-26T03:38:50","password":"","slug":"%e5%88%a9%e7%94%a8%e5%ae%9e%e6%97%b6%e8%8d%a7%e5%85%89%e5%ae%9a%e9%87%8fpcr%e5%92%8c%e6%95%b0%e5%ad%97pcr%e6%a3%80%e6%b5%8b%e9%89%b4%e5%ae%9a%e6%b0%b4%e7%a8%bb%e7%bb%86%e8%8f%8c%e6%80%a7%e6%9d%a1","status":"publish","type":"docs","link":"https:\/\/vibrojet-tech.com\/?docs=%e5%88%a9%e7%94%a8%e5%ae%9e%e6%97%b6%e8%8d%a7%e5%85%89%e5%ae%9a%e9%87%8fpcr%e5%92%8c%e6%95%b0%e5%ad%97pcr%e6%a3%80%e6%b5%8b%e9%89%b4%e5%ae%9a%e6%b0%b4%e7%a8%bb%e7%bb%86%e8%8f%8c%e6%80%a7%e6%9d%a1","title":{"rendered":"\u5229\u7528\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u548c\u6570\u5b57PCR\u68c0\u6d4b\u9274\u5b9a\u6c34\u7a3b\u7ec6\u83cc\u6027\u6761\u6591\u75c5\u83cc"},"content":{"rendered":"\n
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<\/a>\u300a\u6d59\u6c5f\u5927\u5b66\u5b66\u62a5\uff08\u519c\u4e1a\u4e0e\u751f\u547d\u79d1\u5b66\u7248\uff09\u300b<\/p>\n\n\n\n

2023\u5e74\u7b2c1\u671f<\/p>\n<\/div>\n\n\n\n

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DOI:<\/p>\n\n\n\n

<\/a><\/a>10.3785\/j.issn.1008-9209.2021.12.271<\/p>\n<\/div>\n\n\n\n

\u5f20\u5065\u7537 1<\/sup>\uff0c\u738b\u4f9d\u540d 1<\/sup>\uff0c\u5f20\u6d01\u51c0 1<\/sup>\uff0c\u9648\u78ca 2<\/sup>\uff0c\u7f57\u91d1\u71d5 2<\/sup>\uff0c\u6613\u5efa\u5e73 3<\/sup>\uff0c\u674e\u658c 1<\/sup>\uff0c\u5b89\u5343\u91cc 1<\/sup><\/p>\n\n\n\n

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Abstract<\/h2>\n\n\n\n

\u767d\u53f6\u67af\u75c5\u548c\u6761\u6591\u75c5\u662f\u6c34\u7a3b\u91cd\u8981\u7684\u7ec6\u83cc\u6027\u75c5\u5bb3\u3002\u6c34\u7a3b\u767d\u53f6\u67af\u75c5\u83cc (Xanthomonas oryzae<\/em> pv. oryzae<\/em>, Xoo) \u548c\u6c34\u7a3b\u7ec6\u83cc\u6027\u6761\u6591\u75c5\u83cc (Xanthomonas oryzae<\/em> pv. oryzicola<\/em>, Xoc) \u5c5e\u4e8e\u540c\u79cd\u4e0b\u7684 2 \u4e2a\u81f4\u75c5\u53d8\u79cd\u3002\u9274\u522b Xoo \u548c Xoc \u5bf9\u68c0\u75ab\u548c\u9632\u63a7\u8fd9 2 \u79cd\u75c5\u5bb3\u81f3\u5173\u91cd\u8981\u3002Xoo \u548c Xoc \u4e2d\u7684\u94c1 – \u7ea2\u9175\u6bcd\u9178 \/ \u94c1 – \u7caa\u751f\u7d20\u53d7\u4f53\u57fa\u56e0 fhuE<\/em> \u5728\u8fdb\u5316\u8fc7\u7a0b\u4e2d\u56e0\u4e0d\u540c\u7a0b\u5ea6\u5730\u7f3a\u5931\u800c\u6210\u4e3a\u5047\u57fa\u56e0\u3002\u9488\u5bf9 Xoc \u7279\u6709\u800c Xoo \u7f3a\u5931\u7684 fhuE<\/em> \u90e8\u5206\u5e8f\u5217\u8bbe\u8ba1\u5f15\u7269\uff0c\u7b5b\u9009\u83b7\u5f97 Xoc \u7279\u5f02\u6027\u5f15\u7269 XocFhuE-F (5\u2032-ATCGAACGATGTCACCAGGG-3\u2032) \u548c XocFhuE-R (5\u2032-AGAAACGTGCGGCCAGATAA-3\u2032)\u3002\u5229\u7528\u5f15\u7269\u5bf9 XocFhuE-F\/XocFhuE-R \u4ec5\u53ef\u4ece Xoc \u83cc\u682a\u4e2d\u6269\u589e\u51fa 159 bp \u7247\u6bb5\uff0c\u7ed3\u5408\u8367\u5149\u67d3\u6599\u5206\u522b\u5efa\u7acb SYBR Green \u5b9e\u65f6\u8367\u5149\u5b9a\u91cf PCR\uff08quantitative real-time PCR, qPCR\uff09\u4e0e EvaGreen \u5fae\u6ef4\u6570\u5b57 PCR\uff08digital PCR, dPCR\uff09\u68c0\u6d4b\u9274\u5b9a Xoc \u7684\u65b9\u6cd5\u3002\u5728 20 \u03bcL \u53cd\u5e94\u4f53\u7cfb\u4e2d\u52a0\u5165 1 \u03bcL \u6a21\u677f\uff0cqPCR \u68c0\u6d4b\u83cc\u60ac\u6db2\u4e2d Xoc \u7684\u68c0\u51fa\u4e0b\u9650\u4e3a 1.6 \u00d7 10\u2074 CFU\/mL\uff0c\u68c0\u6d4b\u5e26\u83cc\u79cd\u5b50\u4e2d Xoc \u7684\u68c0\u51fa\u4e0b\u9650\u4e3a 1.2 \u00d7 10\u00b3 CFU \/ \u7c92\uff1bdPCR \u68c0\u6d4b\u83cc\u60ac\u6db2\u4e2d Xoc \u7684\u68c0\u51fa\u4e0b\u9650\u4e3a 1.6 \u00d7 10\u00b3 CFU\/mL\uff0c\u68c0\u6d4b\u5e26\u83cc\u79cd\u5b50\u4e2d Xoc \u7684\u68c0\u51fa\u4e0b\u9650\u4e3a 1.2 \u00d7 10\u00b2 CFU \/ \u7c92\u3002\u7efc\u4e0a\u6240\u8ff0\uff0c\u57fa\u4e8e Xoc \u7279\u5f02\u6027\u5f15\u7269 XocFhuE-F\/XocFhuE-R \u5efa\u7acb\u7684 SYBR Green qPCR \u4e0e EvaGreen dPCR \u6280\u672f\uff0c\u53ef\u4e3a\u53e3\u5cb8 Xoc \u68c0\u75ab\u53ca\u6c34\u7a3b\u6761\u6591\u75c5\u76d1\u6d4b\u9884\u8b66\u63d0\u4f9b\u9ad8\u6548\u53ef\u9760\u7684\u68c0\u6d4b\u624b\u6bb5\u3002<\/p>\n\n\n\n

Bacterial leaf blight of rice caused by Xanthomonas oryzae<\/em> pv. oryzae<\/em> (Xoo) and bacterial leaf streak of rice caused by Xanthomonas oryzae<\/em> pv. oryzicola<\/em> (Xoc) are two important bacterial diseases of rice. Identification of Xoo and Xoc belonging to the same species is critical for quarantine and control of these diseases. Here, we found that the fhuE<\/em> gene encoding ferric-rhodotorulic acid\/ferric-coprogen receptor in Xoo and Xoc became pseudogenes after partial deletion during evolution. We designed Xoc-specific primers XocFhuE-F (5\u2032-ATCGAACGATGTCACCAGGG-3\u2032) and XocFhuE-R (5\u2032-AGAAACGTGCGGCCAGATAA-3\u2032) targeting the sequences present in the fhuE<\/em> pseudogene of Xoc but absent in that of Xoo. Polymerase chain reaction (PCR) amplification yielded only a 159 bp DNA fragment from Xoc strains using the primer pair XocFhuE-F\/XocFhuE-R. Based on these Xoc-specific primers, we established SYBR Green quantitative real-time PCR (qPCR) and EvaGreen droplet digital PCR (dPCR) assays for the detection and identification of Xoc. In a 20 \u03bcL reaction system containing 1 \u03bcL template, the limit of detection of qPCR was 1.6 \u00d7 10\u2074 CFU\/mL in bacterial suspensions and 1.2 \u00d7 10\u00b3 CFU\/seed in rice seeds; the limit of detection of dPCR was 1.6 \u00d7 10\u00b3 CFU\/mL in bacterial suspensions and 1.2 \u00d7 10\u00b2 CFU\/seed in rice seeds. In conclusion, SYBR Green qPCR and EvaGreen dPCR established with the Xoc-specific primers XocFhuE-F\/XocFhuE-R are efficient tools for Xoc quarantine and field monitoring of rice bacterial leaf streak.<\/p>\n\n\n\n

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Tag<\/strong>s:<\/h2>\n\n\n\n

\u6c34\u7a3b\u6761\u6591\u75c5\uff08bacterial leaf streak of rice\uff09\uff1b\u6c34\u7a3b\u767d\u53f6\u67af\u75c5\uff08bacterial leaf blight of rice\uff09\uff1b\u6c34\u7a3b\u9ec4\u5355\u80de\u83cc\uff08Xanthomonas oryzae\uff09\uff1b\u4fdd\u5b88\u6807\u5fd7\u86cb\u767d\uff08conserved signature protein\uff09\uff1b\u94c1-\u7caa\u751f\u7d20\u53d7\u4f53\uff08ferric-coprogen receptor\uff09<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Uses qPCR and digital PCR to detect and identify the rice bacterial leaf streak pathogen, demonstrating dPCR applicability in agricultural pathogen testing.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"footnotes":""},"doc_category":[129],"doc_tag":[],"class_list":["post-6614","docs","type-docs","status-publish","hentry","doc_category-broader-research-applications"],"year_month":"2026-05","word_count":308,"total_views":0,"reactions":{"happy":0,"normal":0,"sad":0},"author_info":{"name":"root","author_nicename":"root","author_url":"https:\/\/vibrojet-tech.com\/?author=1"},"doc_category_info":[{"term_name":"Broader Research Applications","term_url":"https:\/\/vibrojet-tech.com\/?doc_category=broader-research-applications"}],"doc_tag_info":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/6614","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs"}],"about":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/types\/docs"}],"author":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6614"}],"version-history":[{"count":6,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/6614\/revisions"}],"predecessor-version":[{"id":14875,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/6614\/revisions\/14875"}],"wp:attachment":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6614"}],"wp:term":[{"taxonomy":"doc_category","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_category&post=6614"},{"taxonomy":"doc_tag","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_tag&post=6614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}