{"id":14123,"date":"2026-05-18T08:53:10","date_gmt":"2026-05-18T00:53:10","guid":{"rendered":"https:\/\/vibrojet-tech.com\/?post_type=docs&p=14123"},"modified":"2026-05-25T17:44:10","modified_gmt":"2026-05-25T09:44:10","password":"","slug":"establishment-of-pseudovirus-reference-material-for-human-norovirus-gii-4-2","status":"publish","type":"docs","link":"https:\/\/vibrojet-tech.com\/?docs=establishment-of-pseudovirus-reference-material-for-human-norovirus-gii-4-2","title":{"rendered":"Establishment of pseudovirus reference material for human norovirus GII.4"},"content":{"rendered":"\n
\n
\n

Microchemical Journal. <\/p>\n\n\n\n

2025 Jun<\/p>\n<\/div>\n\n\n\n

\n

DOI:<\/p>\n\n\n\n

10.1016\/j.microc.2025.113779<\/p>\n<\/div>\n\n\n\n

Jiayi Yang, Mingwei Liu, Yunhua Gao, Huijie Li, Lianhua Dong<\/p>\n\n\n\n

\n

Highlights<\/h2>\n\n\n\n

\u2022 The RT-dPCR method was established to accurately quantify the norovirus G\u2161.4 pseudovirus RM.<\/p>\n\n\n\n

\u2022 The norovirus G\u2161.4 pseudovirus RM containing ORF2 RNA exhibits good homogeneity and stability.<\/p>\n\n\n\n

\u2022 The value-assigned pseudovirus RM can serve as a reliable quality control tool for norovirus G\u2161.4 detection assays.<\/p>\n<\/div>\n\n\n\n

Abstract<\/h2>\n\n\n\n

The human norovirus (NV) is widely recognized as a pathogen causing acute gastroenteritis, particularly associated with elevated morbidity and mortality rates in pediatric and geriatric populations. While current detection methods, such as those based on immune reactions and quantitative polymerase chain reaction (qPCR), have been developed by researchers, these techniques do not provide absolute quantification. In this study, we established a reference material (RM) comprising pseudovirus particles containing NV ORF2 RNA. The RM was quantified using reverse transcription digital PCR (RT-dPCR), demonstrating ideal homogeneity and stability. The assigned value of the RM, including expanded uncertainty, was determined to be (3.42 \u00b1 0.69) \u00d7 10\u00b3 copies\/\u03bcL. This RM can be effectively utilized to validate commercial diagnostic kits and platforms, thereby enhancing the accuracy and reliability of acute gastroenteritis diagnosis.<\/p>\n\n\n\n

\n

Tag<\/strong>s:<\/h2>\n\n\n\n

Human Norovirus G\u2161.4; Acute Gastroenteritis; RT-dPCR; Reference Material<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Assigns values to a human norovirus GII.4 pseudovirus reference material using RT-dPCR and evaluates homogeneity and stability, supporting nucleic acid test quality control.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"footnotes":""},"doc_category":[31],"doc_tag":[],"class_list":["post-14123","docs","type-docs","status-publish","hentry","doc_category-standardization-reference-materials"],"year_month":"2026-05","word_count":195,"total_views":0,"reactions":{"happy":0,"normal":0,"sad":0},"author_info":{"name":"root","author_nicename":"root","author_url":"https:\/\/vibrojet-tech.com\/?author=1"},"doc_category_info":[{"term_name":"Standardization & Reference Materials","term_url":"https:\/\/vibrojet-tech.com\/?doc_category=standardization-reference-materials"}],"doc_tag_info":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/14123","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs"}],"about":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/types\/docs"}],"author":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14123"}],"version-history":[{"count":4,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/14123\/revisions"}],"predecessor-version":[{"id":14815,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/14123\/revisions\/14815"}],"wp:attachment":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14123"}],"wp:term":[{"taxonomy":"doc_category","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_category&post=14123"},{"taxonomy":"doc_tag","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_tag&post=14123"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}