{"id":13919,"date":"2026-05-14T10:04:08","date_gmt":"2026-05-14T02:04:08","guid":{"rendered":"https:\/\/vibrojet-tech.com\/?post_type=docs&p=13919"},"modified":"2026-05-26T14:07:34","modified_gmt":"2026-05-26T06:07:34","password":"","slug":"%e9%ab%98%e7%81%b5%e6%95%8f%e5%be%ae%e6%bb%b4%e5%bc%8f%e6%95%b0%e5%ad%97pcr%e6%a3%80%e6%b5%8b%e4%bd%8e%e6%b0%b4%e5%b9%b3jak2-v617f%e7%aa%81%e5%8f%98","status":"publish","type":"docs","link":"https:\/\/vibrojet-tech.com\/?docs=%e9%ab%98%e7%81%b5%e6%95%8f%e5%be%ae%e6%bb%b4%e5%bc%8f%e6%95%b0%e5%ad%97pcr%e6%a3%80%e6%b5%8b%e4%bd%8e%e6%b0%b4%e5%b9%b3jak2-v617f%e7%aa%81%e5%8f%98","title":{"rendered":"\u9ad8\u7075\u654f\u5fae\u6ef4\u5f0f\u6570\u5b57 PCR \u68c0\u6d4b\u4f4e\u6c34\u5e73 JAK2 V617F \u7a81\u53d8"},"content":{"rendered":"\n
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\u300a\u4e2d\u56fd\u751f\u7269\u5de5\u7a0b\u6742\u5fd7\u300b<\/p>\n\n\n\n

2023\u5e74\u7b2c12\u671f<\/p>\n<\/div>\n\n\n\n

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DOI:<\/p>\n\n\n\n

<\/a>10.13523\/j.cb.2310057<\/p>\n<\/div>\n\n\n\n

\u9f99\u73b2\u7389\uff0c\u5468\u4e9a\u5170\uff0c\u7126\u7476\uff0c\u53f6\u6797\u6d77\uff0c\u674e\u4fee\u6625\uff0c\u5218\u8273\u8363\uff0c\u9ec4\u6653\u519b\uff0c\u962e\u56fd\u745e<\/p>\n\n\n\n

\n\n\n\n

Abstract<\/h2>\n\n\n\n
\n
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\u76ee\u7684\uff1a<\/p>\n\n\n\n

\u5efa\u7acb\u4e00\u79cd\u68c0\u6d4b\u4f4e\u6c34\u5e73 JAK2<\/em> V617F \u7a81\u53d8\u7684\u5fae\u6ef4\u5f0f\u6570\u5b57 PCR (droplet digital PCR,ddPCR) \u65b9\u6cd5\u5e76\u63a2\u8ba8\u5176\u5728\u9aa8\u9ad3\u589e\u6b96\u6027\u80bf\u7624 (myeloproliferative neoplasm,MPN) \u4e2d\u7684\u5e94\u7528\u4ef7\u503c\u3002<\/p>\n<\/div>\n\n\n\n

\n

\u65b9\u6cd5\uff1a<\/p>\n\n\n\n

\u5229\u7528\u4f4d\u70b9\u7279\u5f02\u6027\u7684 TaqMan-MGB \u63a2\u9488\uff0c\u5efa\u7acb\u7528\u4e8e\u68c0\u6d4b\u57fa\u56e0\u7ec4 DNA \u4e2d JAK2<\/em> V617F \u7a81\u53d8\u7684 ddPCR \u65b9\u6cd5\uff0c\u5e94\u7528\u8be5\u65b9\u6cd5\u3001\u5b9e\u65f6\u5b9a\u91cf PCR \u53ca\u4e8c\u4ee3\u6d4b\u5e8f\u65b9\u6cd5\u68c0\u6d4b MPN \u60a3\u8005 JAK2<\/em> V617F \u7a81\u53d8\uff0c\u6bd4\u8f83\u4e0d\u540c\u65b9\u6cd5\u68c0\u6d4b\u7ed3\u679c\u7684\u4e00\u81f4\u6027\u3002<\/p>\n<\/div>\n\n\n\n

\n

\u7ed3\u679c\uff1a<\/p>\n\n\n\n

\u6210\u529f\u5efa\u7acb\u4e86\u4e00\u79cd\u57fa\u4e8e\u7279\u5f02\u6027 TaqMan-MGB \u63a2\u9488\u68c0\u6d4b JAK2<\/em> V617F \u7a81\u53d8\u7684 ddPCR \u65b9\u6cd5\uff0c\u68c0\u6d4b\u7075\u654f\u5ea6\u81f3\u5c11\u4e3a 0.05 %\u3002\u5e94\u7528 ddPCR \u65b9\u6cd5\u53ca\u5b9e\u65f6\u5b9a\u91cf PCR \u65b9\u6cd5\u5bf9 82 \u4efd MPN \u60a3\u8005\u53ca 8 \u4efd\u5065\u5eb7\u5bf9\u7167\u8005\u7684\u9aa8\u9ad3\u6216\u5916\u5468\u8840 DNA \u6837\u672c\u540c\u65f6\u8fdb\u884c\u68c0\u6d4b\uff0c\u5176\u4e2d 87 \u4efd\u6837\u672c\u68c0\u6d4b\u7ed3\u679c\u4e00\u81f4\uff0c\u68c0\u51fa\u7684\u4e00\u81f4\u7387\u4e3a 96.7 %\uff1b3 \u4efd\u7ecf\u5b9e\u65f6\u5b9a\u91cf PCR \u65b9\u6cd5\u68c0\u6d4b\u4e3a\u9634\u6027\u800c ddPCR \u68c0\u6d4b\u4e3a\u9633\u6027\u7684\u6837\u672c\uff0cddPCR \u7684\u5b9a\u91cf\u7ed3\u679c\u5206\u522b\u4e3a 0.015 %\u30010.002 %\u30010.039 %, \u5747\u4f4e\u4e8e\u5b9e\u65f6\u5b9a\u91cf PCR \u7684\u68c0\u51fa\u4e0b\u9650\uff1b\u5bf9\u4e8e\u4e24\u79cd\u65b9\u6cd5\u68c0\u6d4b\u7ed3\u679c\u5747\u4e3a\u9633\u6027\u7684 52 \u4efd\u6837\u672c\uff0c\u5176\u5b9a\u91cf\u6570\u636e\u7684\u76f8\u5173\u7cfb\u6570\u4e3a 0.9714 (P<\/em> < 0.0001)\u300211 \u4f8b\u6837\u672c\u540c\u65f6\u91c7\u7528\u4e8c\u4ee3\u6d4b\u5e8f\u7684\u65b9\u6cd5\u8fdb\u884c\u9a8c\u8bc1\uff0c\u4e24\u8005\u5b9a\u91cf\u7ed3\u679c\u7684\u76f8\u5173\u7cfb\u6570\u4e3a 0.9839 (P<\/em> < 0.0001)\u3002<\/p>\n<\/div>\n\n\n\n

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\u7ed3\u8bba\uff1a<\/p>\n\n\n\n

\u5229\u7528\u7279\u5f02\u6027 TaqMan-MGB \u63a2\u9488\u68c0\u6d4b JAK2<\/em> V617F \u7a81\u53d8\u7684\u5fae\u6ef4\u5f0f\u6570\u5b57 PCR \u65b9\u6cd5\u53ef\u4fbf\u6377\u3001\u51c6\u786e\u5730\u68c0\u6d4b JAK2<\/em> V617F \u7a81\u53d8\uff0c\u8be5\u65b9\u6cd5\u5177\u6709\u9ad8\u5ea6\u7684\u7075\u654f\u5ea6\u4e14\u53ef\u7edd\u5bf9\u5b9a\u91cf\uff0c\u5728 JAK2<\/em> V617F \u7a81\u53d8\u7684\u7b5b\u67e5\u53ca\u52a8\u6001\u76d1\u6d4b\u4e2d\u5177\u6709\u6f5c\u5728\u5e94\u7528\u4ef7\u503c\u3002<\/p>\n<\/div>\n\n\n\n

\n

Objective: <\/p>\n\n\n\n

To establish a droplet digital PCR (ddPCR) method for detecting the low-level JAK2<\/em> V617F mutation and to explore its application value in the diagnosis of myeloproliferative neoplasm (MPN).<\/p>\n<\/div>\n\n\n\n

\n

Methods:\u00a0<\/p>\n\n\n\n

Site-specific TaqMan-MGB probes were used to establish the ddPCR method for detecting the JAK2<\/em> V617F mutation in genomic DNA. JAK2<\/em> V617F mutations were detected by ddPCR, real-time quantitative PCR and next-generation sequencing (NGS). The consistency of the detection results of different methods was compared.<\/p>\n<\/div>\n\n\n\n

\n

Results:\u00a0<\/p>\n\n\n\n

The ddPCR method for detecting the JAK2<\/em> V617F mutation based on specific TaqMan-MGB probes was successfully established, and the detection sensitivity was at least 0.05 %. The ddPCR method and qPCR method were used to detect the JAK2<\/em> V617F mutation in 82 subjects with MPN and 8 healthy controls. The consistency rate was 96.7 %. For the 52 samples with positive results by both methods, the correlation coefficient of quantitative data was 0.9714 (P<\/em> < 0.0001). Eleven samples were verified by NGS and the correlation coefficient between the two quantitative results was 0.9839 (P<\/em> < 0.0001).<\/p>\n<\/div>\n\n\n\n

\n

Conclusion:\u00a0<\/p>\n\n\n\n

The ddPCR with the specific TaqMan-MGB probe method can detect the JAK2<\/em> V617F mutation conveniently and accurately, which may have potential application value in the screening and dynamic monitoring of the JAK2<\/em> V617F mutation.<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n

Tag<\/strong>s:<\/h2>\n\n\n\n

\u9aa8\u9ad3\u589e\u6b96\u6027\u80bf\u7624\uff08Myeloproliferative neoplasms\uff09\uff1bJAK2 V617F\u7a81\u53d8\uff08JAK2 V617F mutation\uff09\uff1b \u5fae\u6ef4\u5f0f\u6570\u5b57PCR\uff08Droplet digital PCR\uff09<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Uses highly sensitive ddPCR to detect low-level JAK2 V617F mutation, supporting the clinical-translational value of dPCR for low-abundance hematologic mutation monitoring.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"footnotes":""},"doc_category":[32],"doc_tag":[],"class_list":["post-13919","docs","type-docs","status-publish","hentry","doc_category-clinical-translational-evidence"],"year_month":"2026-05","word_count":271,"total_views":0,"reactions":{"happy":0,"normal":0,"sad":0},"author_info":{"name":"root","author_nicename":"root","author_url":"https:\/\/vibrojet-tech.com\/?author=1"},"doc_category_info":[{"term_name":"Clinical & Translational Evidence","term_url":"https:\/\/vibrojet-tech.com\/?doc_category=clinical-translational-evidence"}],"doc_tag_info":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13919","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs"}],"about":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/types\/docs"}],"author":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13919"}],"version-history":[{"count":5,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13919\/revisions"}],"predecessor-version":[{"id":14895,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13919\/revisions\/14895"}],"wp:attachment":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13919"}],"wp:term":[{"taxonomy":"doc_category","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_category&post=13919"},{"taxonomy":"doc_tag","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_tag&post=13919"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}