{"id":13888,"date":"2026-05-13T17:20:09","date_gmt":"2026-05-13T09:20:09","guid":{"rendered":"https:\/\/vibrojet-tech.com\/?post_type=docs&p=13888"},"modified":"2026-05-25T17:20:12","modified_gmt":"2026-05-25T09:20:12","password":"","slug":"%e9%9c%8d%e4%b9%b1%e5%bc%a7%e8%8f%8c%e6%a0%b8%e9%85%b8%e6%a3%80%e6%b5%8b%e8%af%95%e5%89%82%e5%9b%bd%e5%ae%b6%e5%8f%82%e8%80%83%e5%93%81%e7%9a%84%e5%bb%ba%e7%ab%8b","status":"publish","type":"docs","link":"https:\/\/vibrojet-tech.com\/?docs=%e9%9c%8d%e4%b9%b1%e5%bc%a7%e8%8f%8c%e6%a0%b8%e9%85%b8%e6%a3%80%e6%b5%8b%e8%af%95%e5%89%82%e5%9b%bd%e5%ae%b6%e5%8f%82%e8%80%83%e5%93%81%e7%9a%84%e5%bb%ba%e7%ab%8b","title":{"rendered":"\u970d\u4e71\u5f27\u83cc\u6838\u9178\u68c0\u6d4b\u8bd5\u5242\u56fd\u5bb6\u53c2\u8003\u54c1\u7684\u5efa\u7acb"},"content":{"rendered":"\n
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\u300a\u5206\u5b50\u8bca\u65ad\u4e0e\u6cbb\u7597\u6742\u5fd7\u300b<\/p>\n\n\n\n

2025\u5e74\u7b2c2\u671f<\/p>\n<\/div>\n\n\n\n

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DOI:<\/p>\n\n\n\n

<\/a>10.3969\/j.issn.1674-6929.2025.02.002<\/p>\n<\/div>\n\n\n\n

\u9ebb\u5a77\u5a77\u00a01<\/sup>\uff0c\u5218\u98de\u00a02<\/sup>\uff0c\u80e1\u664b\u541b\u00a01<\/sup>\uff0c\u8bb8\u56db\u5b8f\u00a01<\/sup>\uff0c\u5468\u6d77\u536b\u00a01<\/sup><\/p>\n\n\n\n

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Abstract<\/h2>\n\n\n\n
\n
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\u76ee\u7684\uff1a<\/p>\n\n\n\n

\u5efa\u7acb\u970d\u4e71\u5f27\u83cc\u6838\u9178\u68c0\u6d4b\u8bd5\u5242\u56fd\u5bb6\u53c2\u8003\u54c1\u5e76\u5236\u5b9a\u8d28\u91cf\u6807\u51c6\u3002<\/p>\n<\/div>\n\n\n\n

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\u65b9\u6cd5\uff1a<\/strong><\/p>\n\n\n\n

\u6536\u96c6\u5e76\u7b5b\u9009\u970d\u4e71\u5f27\u83cc\u6838\u9178\u9633\u6027\u548c\u9634\u6027\u7ec6\u83cc\u57f9\u517b\u7269\u6837\u672c\uff0c\u5efa\u7acb\u970d\u4e71\u5f27\u83cc\u6838\u9178\u68c0\u6d4b\u8bd5\u5242\u56fd\u5bb6\u53c2\u8003\u54c1\uff0c\u5e76\u5bf9\u53c2\u8003\u54c1\u7a33\u5b9a\u6027\u548c\u5747\u5300\u6027\u8fdb\u884c\u8bc4\u4ef7\uff1b\u7ecf 7 \u4e2a\u5b9e\u9a8c\u5ba4\u7684\u534f\u4f5c\u6807\u5b9a\uff0c\u4f9d\u636e\u6807\u5b9a\u7ed3\u679c\u5236\u5b9a\u56fd\u5bb6\u53c2\u8003\u54c1\u8d28\u91cf\u6807\u51c6\u3002<\/p>\n<\/div>\n\n\n\n

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\u7ed3\u679c\uff1a<\/strong><\/p>\n\n\n\n

\u970d\u4e71\u5f27\u83cc\u6838\u9178\u68c0\u6d4b\u8bd5\u5242\u56fd\u5bb6\u53c2\u8003\u54c1\u5305\u62ec\u9633\u6027 8 \u4efd\u3001\u9634\u6027 10 \u4efd\u3001\u91cd\u590d\u6027 2 \u4efd\u548c\u68c0\u6d4b\u9650 2 \u4efd\u3002\u53c2\u8003\u54c1\u5747\u5300\u6027\u7b26\u5408\u8981\u6c42\u3002\u51bb\u878d 1 \u6b21\u30012\u20138 \u2103\u53ca\u5ba4\u6e29\uff0825 \u2103\uff09\u73af\u5883\u4e2d\u653e\u7f6e 7 \u5929\u5747\u4e0d\u5f71\u54cd\u53c2\u8003\u54c1\u7684\u7a33\u5b9a\u6027\u3002\u56fd\u5bb6\u53c2\u8003\u54c1\u7684\u8d28\u91cf\u6807\u51c6\u4e3a\u9633\u6027\u7b26\u5408\u7387 8\/8\uff0c\u9634\u6027\u7b26\u5408\u7387 10\/10\uff0c\u91cd\u590d\u4e24\u4e2a\u6d53\u5ea6\u6c34\u5e73 Ct \u503c\u7684\u53d8\u5f02\u7cfb\u6570\u5747\u4e0d\u9ad8\u4e8e 5.0 %\uff0cL1 \u548c L2 \u8981\u6c42 1:104<\/sup> \u7a00\u91ca\u6216\u66f4\u9ad8\u6d53\u5ea6\u65f6\u5e94\u5747\u4e3a\u970d\u4e71\u5f27\u83cc\u9633\u6027\u3002\u68c0\u6d4b\u9650\u548c\u91cd\u590d\u6027\u53c2\u8003\u54c1 L1\u3001L2\u3001R1 \u548c R2 \u5206\u522b\u8d4b\u503c\u4e3a 3.9\u00d710\u2077 U\/mL\u30012.4\u00d710\u2077 U\/mL\u30017.3\u00d710\u2077 U\/mL \u548c 6.3\u00d710\u2077 U\/mL\u3002<\/p>\n<\/div>\n\n\n\n

\n

\u7ed3\u8bba\uff1a<\/strong><\/p>\n\n\n\n

\u5efa\u7acb\u4e86\u970d\u4e71\u5f27\u83cc\u6838\u9178\u68c0\u6d4b\u8bd5\u5242\u56fd\u5bb6\u53c2\u8003\u54c1\uff0c\u53ef\u7528\u4e8e\u76f8\u5173\u8bd5\u5242\u7684\u8d28\u91cf\u63a7\u5236\u53ca\u8bc4\u4ef7\u3002<\/p>\n<\/div>\n\n\n\n

\n

Objective: <\/p>\n\n\n\n

To develop a national reference panel for Vibrio cholerae nucleic acid tests and establish the relative quality requirements.<\/p>\n<\/div>\n\n\n\n

\n

Methods: <\/strong><\/p>\n\n\n\n

The bacterium culture samples positive and negative for Vibrio cholerae nucleic acid were collected and screened to establish a national reference panel for Vibrio cholerae nucleic acid tests. The stability and homogeneity of the reference panel were evaluated, and the quality criteria were determined via collaborative calibration conducted by seven laboratories.<\/p>\n<\/div>\n\n\n\n

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Results: <\/strong><\/p>\n\n\n\n

The national reference panel consists of 8 positive, 10 negative, 2 precision and 2 lower detection limit samples. The homogeneity of the reference panel meets the requirements, and the stability of the reference panel was not affected by freezing and thawing once, storing for 7 days at 2\u20138 \u2103 and room temperature (25 \u2103). The quality requirements of the national reference panel are that the positive coincidence rate is 8\/8, and the negative coincidence rate is 10\/10. For precision, the coefficient of variation (CV) of the Ct value at the two concentration levels is not higher than 5.0 %. For the limit of minimum detection, a dilution ratio of 1\u223610\u2074 or higher concentrations on L1 and L2 should be positive for nucleic acid of Vibrio cholerae. Precision and lower detection limit samples L1, L2, R1 and R2 are assigned as 9\u00d710\u2077 U\/mL, 2.4\u00d710\u2077 U\/mL, 7.3\u00d710\u2077 U\/mL, and 6.3\u00d710\u2077 U\/mL.<\/p>\n<\/div>\n\n\n\n

\n

Conclusion: <\/strong><\/p>\n\n\n\n

A national reference panel for Vibrio cholerae nucleic acid tests has been established. This panel can be used for quality control and evaluation of related diagnostics.<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n

Tag<\/strong>s:<\/h2>\n\n\n\n

\u970d\u4e71\u5f27\u83cc\uff08Vibrio cholera\uff09\uff1b\u6838\u9178\u68c0\u6d4b\uff08Nucleic acid tests\uff09\uff1b \u53c2\u8003\u54c1\uff08Reference panel\uff09\uff1b\u8d28\u91cf\u8bc4\u4ef7\uff08Quality requirements\uff09<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Establishes a national reference panel for Vibrio cholerae nucleic acid test reagents, supporting quality evaluation and standardization of related IVD assays.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"footnotes":""},"doc_category":[31],"doc_tag":[],"class_list":["post-13888","docs","type-docs","status-publish","hentry","doc_category-standardization-reference-materials"],"year_month":"2026-05","word_count":268,"total_views":0,"reactions":{"happy":0,"normal":0,"sad":0},"author_info":{"name":"root","author_nicename":"root","author_url":"https:\/\/vibrojet-tech.com\/?author=1"},"doc_category_info":[{"term_name":"Standardization & Reference Materials","term_url":"https:\/\/vibrojet-tech.com\/?doc_category=standardization-reference-materials"}],"doc_tag_info":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13888","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs"}],"about":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/types\/docs"}],"author":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13888"}],"version-history":[{"count":4,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13888\/revisions"}],"predecessor-version":[{"id":14808,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13888\/revisions\/14808"}],"wp:attachment":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13888"}],"wp:term":[{"taxonomy":"doc_category","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_category&post=13888"},{"taxonomy":"doc_tag","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_tag&post=13888"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}