{"id":13881,"date":"2026-05-13T17:13:22","date_gmt":"2026-05-13T09:13:22","guid":{"rendered":"https:\/\/vibrojet-tech.com\/?post_type=docs&p=13881"},"modified":"2026-05-25T15:50:35","modified_gmt":"2026-05-25T07:50:35","password":"","slug":"%e9%9d%b6%e5%90%91%e8%8d%af%e7%89%a9%e7%94%b2%e7%a3%ba%e9%85%b8%e4%bc%8a%e9%a9%ac%e6%9b%bf%e5%b0%bc%e7%9a%84%e5%88%86%e5%ad%90%e6%a0%87%e5%bf%97%e7%89%a9bcr-abl%e8%9e%8d%e5%90%88%e5%9f%ba%e5%9b%a0","status":"publish","type":"docs","link":"https:\/\/vibrojet-tech.com\/?docs=%e9%9d%b6%e5%90%91%e8%8d%af%e7%89%a9%e7%94%b2%e7%a3%ba%e9%85%b8%e4%bc%8a%e9%a9%ac%e6%9b%bf%e5%b0%bc%e7%9a%84%e5%88%86%e5%ad%90%e6%a0%87%e5%bf%97%e7%89%a9bcr-abl%e8%9e%8d%e5%90%88%e5%9f%ba%e5%9b%a0","title":{"rendered":"\u9776\u5411\u836f\u7269\u7532\u78fa\u9178\u4f0a\u9a6c\u66ff\u5c3c\u7684\u5206\u5b50\u6807\u5fd7\u7269 BCR-ABL \u878d\u5408\u57fa\u56e0\u5b9a\u91cf\u68c0\u6d4b\u5e73\u53f0\u7684\u8d28\u91cf\u8bc4\u4ef7\u65b9\u6cd5\u7684\u7814\u7a76"},"content":{"rendered":"\n
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\u300a\u4e2d\u56fd\u836f\u4e8b\u300b<\/p>\n\n\n\n

2024\u5e741\u671f<\/p>\n<\/div>\n\n\n\n

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DOI:<\/p>\n\n\n\n

<\/a>10.16153\/j.1002-7777.2024.01.008<\/p>\n<\/div>\n\n\n\n

\u5b59\u6960\uff0c\u674e\u4e3d\u8389\uff0c\u5f20\u6587\u65b0\uff0c\u9ec4\u6770\uff0c\u66f2\u5b88\u65b9<\/p>\n\n\n\n

\n\n\n\n

Abstract<\/h2>\n\n\n\n
\n
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\u76ee\u7684\uff1a<\/p>\n\n\n\n

\u4f7f\u7528 BCR-ABL \u5b9a\u91cf\u6807\u51c6\u54c1\uff0c\u8bc4\u4ef7 BCR-ABL \u878d\u5408\u57fa\u56e0\u68c0\u6d4b\u8bd5\u5242\u76d2 (\u6570\u5b57 PCR \u6cd5) \u7684\u6027\u80fd\u3002<\/p>\n<\/div>\n\n\n\n

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\u65b9\u6cd5\uff1a<\/strong><\/p>\n\n\n\n

\u63d0\u53d6 BCR-ABL \u5b9a\u91cf\u6807\u51c6\u54c1\u7684 RNA\uff0c\u6d4b\u5b9a\u5176\u6d53\u5ea6\u548c\u7eaf\u5ea6\u3002\u91c7\u7528\u8be5\u8bd5\u5242\u76d2\u642d\u914d\u6570\u5b57 PCR \u4eea\u68c0\u6d4b\uff0c\u83b7\u53d6\u6807\u51c6\u54c1 BCR-ABL \u878d\u5408\u57fa\u56e0\u5206\u5b50\u5b66\u53cd\u5e94\u7ed3\u679c\u3002<\/p>\n<\/div>\n\n\n\n

\n

\u7ed3\u679c\uff1a<\/strong><\/p>\n\n\n\n

\u51c6\u786e\u5ea6\u68c0\u6d4b\u6837\u54c1 WS2\u3001WS3 \u7684 BCR-ABL \u878d\u5408\u57fa\u56e0 MR \u7edd\u5bf9\u504f\u5dee\u5747\u4e0d\u8d85\u8fc7\u00b10.5 log\uff1b\u68c0\u51fa\u9650\u6837\u54c1 WS4 \u53ef\u68c0\u51fa\u878d\u5408\u57fa\u56e0\u9633\u6027\uff1b\u91cd\u590d\u6027\u6837\u54c1 WS1\u3001WS4 \u5bf9\u5e94\u7684 MR \u53d8\u5f02\u7cfb\u6570\u5747\u5c0f\u4e8e3.0 %\u3002<\/p>\n<\/div>\n\n\n\n

\n

\u7ed3\u8bba\uff1a<\/strong><\/p>\n\n\n\n

\u8be5\u8bd5\u5242\u76d2\u51c6\u786e\u5ea6\u3001\u68c0\u51fa\u9650\u3001\u91cd\u590d\u6027\u6307\u6807\u5747\u7b26\u5408\u300a\u65ad\u88c2\u70b9\u7c07\u96c6\u533a – \u827e\u8d1d\u5c14\u900a\u767d\u8840\u75c5\u75c5\u6bd2 (BCR-ABL) \u878d\u5408\u57fa\u56e0\u68c0\u6d4b\u8bd5\u5242\u76d2\u300b\u76f8\u5173\u6807\u51c6\u8981\u6c42\uff0c\u53ef\u4e3a\u6807\u51c6\u843d\u5730\u5b9e\u65bd\u63d0\u4f9b\u6280\u672f\u652f\u6491\u3002<\/p>\n<\/div>\n\n\n\n

\n

Objective: <\/p>\n\n\n\n

To evaluate performance of BCR-ABL fusion gene testing kit by digital PCR method using BCR-ABL quantitative standard.<\/p>\n<\/div>\n\n\n\n

\n

Methods: <\/strong><\/p>\n\n\n\n

The RNA of standard was extracted and its concentration and purity was determined. The BCR-ABL fusion gene testing kit (digital PCR method) and digital PCR instrument were adopted for detection, and the molecular response level of BCR-ABL fusion gene of the quantitative standard was obtained.<\/p>\n<\/div>\n\n\n\n

\n

Results: <\/strong><\/p>\n\n\n\n

The MR absolute deviation of accuracy standards WS2 and WS3 did not exceed \u00b10.5 log. The detection limit standard WS4 showed positive results of BCR-ABL fusion gene. The coefficient of variation (CV) of MR for repeatability standards WS1 and WS4 was less than 3.0 %.<\/p>\n<\/div>\n\n\n\n

\n

Conclusion: <\/strong><\/p>\n\n\n\n

The performance indicators including accuracy, detection limit and repeatability of the BCR-ABL fusion gene quantitative testing kit (digital PCR method) meet relevant standard requirements, providing technical support for standard implementation.<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n

Tag<\/strong>s:<\/h2>\n\n\n\n

\u65ad\u88c2\u70b9\u7c07\u96c6\u533a-\u827e\u8d1d\u5c14\u900a\u767d\u8840\u75c5\u75c5\u6bd2\u878d\u5408\u57fa\u56e0\uff08Breakpoint cluster region-Abelson leukemia virus fusion gene\uff09\uff1b\u56fd\u9645\u6807\u51c6\u5316\uff08international standardization\uff09\uff1b\u8f6c\u5316\u7cfb\u6570\uff08conversion factor\uff09\uff1b\u5206\u5b50\u5b66\u53cd\u5e94\uff08molecular response\uff09\uff1b\u51c6\u786e\u5ea6\uff08accuracy\uff09\uff1b\u68c0\u51fa\u9650\uff08detection limit\uff09\uff1b\u91cd\u590d\u6027\uff08repeatability\uff09\uff1b\u6eaf\u6e90\uff08traceability\uff09<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Develops a quality-evaluation approach for BCR-ABL fusion gene quantification platforms related to imatinib therapy, supporting standardization and comparability in CML molecular monitoring.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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