{"id":13850,"date":"2026-05-13T14:54:31","date_gmt":"2026-05-13T06:54:31","guid":{"rendered":"https:\/\/vibrojet-tech.com\/?post_type=docs&p=13850"},"modified":"2026-05-25T15:24:25","modified_gmt":"2026-05-25T07:24:25","password":"","slug":"%e5%be%ae%e6%bb%b4%e5%bc%8f%e6%95%b0%e5%ad%97pcr%e5%ae%9a%e9%87%8f%e6%a3%80%e6%b5%8b%e4%bc%af%e6%b0%8f%e8%80%83%e5%85%8b%e6%96%af%e6%b0%8f%e4%bd%93%e6%96%b9%e6%b3%95%e7%9a%84%e5%bb%ba%e7%ab%8b","status":"publish","type":"docs","link":"https:\/\/vibrojet-tech.com\/?docs=%e5%be%ae%e6%bb%b4%e5%bc%8f%e6%95%b0%e5%ad%97pcr%e5%ae%9a%e9%87%8f%e6%a3%80%e6%b5%8b%e4%bc%af%e6%b0%8f%e8%80%83%e5%85%8b%e6%96%af%e6%b0%8f%e4%bd%93%e6%96%b9%e6%b3%95%e7%9a%84%e5%bb%ba%e7%ab%8b","title":{"rendered":"\u5fae\u6ef4\u5f0f\u6570\u5b57 PCR \u5b9a\u91cf\u68c0\u6d4b\u4f2f\u6c0f\u8003\u514b\u65af\u6c0f\u4f53\u65b9\u6cd5\u7684\u5efa\u7acb\u53ca\u5e94\u7528"},"content":{"rendered":"\n
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<\/a>\u300a\u5e7f\u897f\u519c\u5b66\u62a5\u300b<\/p>\n\n\n\n

2024\u5e74\u7b2c2\u671f<\/p>\n<\/div>\n\n\n\n

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DOI:<\/p>\n\n\n\n

<\/a><\/a>10.20160\/j.cnki.ISSN1003-4374.2024.02.005<\/p>\n<\/div>\n\n\n\n

\u738b\u4e9a\u9e4f 1<\/sup>\uff0c\u66f2\u7476 2<\/sup>\uff0c\u5f20\u7693\u535a 3<\/sup>\uff0c\u674e\u5609\u742a 4<\/sup>\uff0c\u7109\u946b 4<\/sup>\uff0c\u5b59\u6dd1\u82b3 3<\/sup>\uff0c\u5b59\u7fd4\u7fd4 4<\/sup>\uff0c\u6b27\u9633\u5eb7 5<\/sup><\/p>\n\n\n\n

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Abstract<\/h2>\n\n\n\n

\u4e3a\u5efa\u7acb\u4f2f\u6c0f\u8003\u514b\u65af\u6c0f\u4f53\uff08Coxiella burnetii, Cb\uff09\u5fae\u6ef4\u5f0f\u6570\u5b57 PCR\uff08Droplet Digital PCR, ddPCR\uff09\u68c0\u6d4b\u65b9\u6cd5\uff0c\u7814\u7a76\u4ee5 Cb IS1111<\/em>\u57fa\u56e0\u4e3a\u9776\u57fa\u56e0\uff0c\u9488\u5bf9\u57fa\u56e0\u4fdd\u5b88\u533a\u57df\u8bbe\u8ba1\u7279\u5f02\u6027\u5f15\u7269\u548c\u63a2\u9488\uff0c\u4f18\u5316\u53cd\u5e94\u6761\u4ef6\u6784\u5efa ddPCR \u68c0\u6d4b\u65b9\u6cd5\uff0c\u5e76\u8bc4\u4f30\u8be5\u65b9\u6cd5\u7684\u7279\u5f02\u6027\u3001\u654f\u611f\u6027\u53ca\u91cd\u590d\u6027\u3002\u7ed3\u679c\u663e\u793a\uff0c\u8be5\u65b9\u6cd5\u6700\u4f4e\u68c0\u51fa\u6d53\u5ea6\u4e3a0.52 copies\/\u03bcL\uff0c\u654f\u611f\u6027\u8f83\u9ad8\uff1b\u4e0e\u52a8\u7269\u5e03\u9c81\u6c0f\u83cc\u3001\u725b\u7ed3\u6838\u5206\u679d\u6746\u83cc\u3001\u571f\u62c9\u6746\u83cc\u3001\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u548c\u6c99\u95e8\u6c0f\u83cc\u65e0\u4ea4\u53c9\u53cd\u5e94\uff0c\u7279\u5f02\u6027\u5f3a\uff1b\u7ec4\u5185\u91cd\u590d\u548c\u7ec4\u95f4\u91cd\u590d\u53d8\u5f02\u7cfb\u6570\u5747\u5c0f\u4e8e10 %\uff0c\u91cd\u590d\u6027\u597d\u3002\u91c7\u7528\u672c\u65b9\u6cd5\u68c0\u6d4b 42 \u4efd\u7591\u4f3c\u611f\u67d3\u4e34\u5e8a\u6837\u54c1\uff0c\u68c0\u6d4b\u7ed3\u679c\u4e0e\u8367\u5149 PCR \u9274\u5b9a\u7ed3\u679c\u4e00\u81f4\u3002\u5efa\u7acb\u7684 ddPCR \u65b9\u6cd5\u7279\u5f02\u6027\u5f3a\u3001\u7075\u654f\u5ea6\u9ad8\u3001\u91cd\u590d\u6027\u4e0e\u51c6\u786e\u6027\u826f\u597d\uff0c\u53ef\u7528\u4e8e Cb \u5feb\u901f\u68c0\u6d4b\u548c\u7cbe\u51c6\u5b9a\u91cf\uff0c\u5bf9 Cb \u611f\u67d3\u9632\u63a7\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002<\/p>\n\n\n\n

In order to establish a Droplet Digital PCR (ddPCR) detection method for Coxiella burnetii<\/em> (Cb), this study designed specific primers and probes targeting the conserved region of the Cb IS1111 gene and optimized reaction conditions. A ddPCR detection assay was established, and its specificity, sensitivity and repeatability were evaluated. The results showed that the limit of detection was 0.52 copies\/\u03bcL, reflecting high sensitivity. No cross-reactivity was observed with animal Brucella<\/em>, Mycobacterium bovis<\/em>, Pseudomonas aeruginosa<\/em>, Staphylococcus aureus<\/em> and Salmonella<\/em>, presenting favorable specificity. Both intra-assay and inter-assay coefficients of variation were lower than 10 %. A total of 42 suspected clinical samples were tested, and the results were consistent with those obtained by fluorescent PCR. The established ddPCR method features high specificity, sensitivity, repeatability and accuracy, and is applicable to rapid detection and absolute quantification of Cb, which facilitates prevention and control of Cb infection.<\/p>\n\n\n\n

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Tag<\/strong>s:<\/h2>\n\n\n\n

\u4f2f\u6c0f\u8003\u514b\u65af\u6c0f\u4f53\uff08Coxiella burnetii\uff09\uff1bQ\u70ed\uff08Q fever\uff09\uff1b\u5fae\u6ef4\u5f0f\u6570\u5b57PCR\uff08Droplet Digital PCR\uff09\uff1b\u4e34\u5e8a\u68c0\u6d4b\uff08clinical detection\uff09<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Establishes a ddPCR method for quantitative detection of Coxiella burnetii, supporting digital PCR potential in low-abundance zoonotic pathogen detection.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"footnotes":""},"doc_category":[30],"doc_tag":[],"class_list":["post-13850","docs","type-docs","status-publish","hentry","doc_category-platform-analytical-performance"],"year_month":"2026-05","word_count":183,"total_views":0,"reactions":{"happy":0,"normal":0,"sad":0},"author_info":{"name":"root","author_nicename":"root","author_url":"https:\/\/vibrojet-tech.com\/?author=1"},"doc_category_info":[{"term_name":"Platform Analytical Performance","term_url":"https:\/\/vibrojet-tech.com\/?doc_category=platform-analytical-performance"}],"doc_tag_info":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13850","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs"}],"about":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/types\/docs"}],"author":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13850"}],"version-history":[{"count":11,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13850\/revisions"}],"predecessor-version":[{"id":14767,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13850\/revisions\/14767"}],"wp:attachment":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13850"}],"wp:term":[{"taxonomy":"doc_category","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_category&post=13850"},{"taxonomy":"doc_tag","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_tag&post=13850"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}