{"id":13846,"date":"2026-05-13T14:50:58","date_gmt":"2026-05-13T06:50:58","guid":{"rendered":"https:\/\/vibrojet-tech.com\/?post_type=docs&p=13846"},"modified":"2026-05-25T15:13:37","modified_gmt":"2026-05-25T07:13:37","password":"","slug":"%e6%96%b0%e5%9e%8b%e5%86%a0%e7%8a%b6%e7%97%85%e6%af%92b-1-1-7%e5%8f%98%e5%bc%82%e6%a0%aa%e7%89%b9%e5%bc%82%e6%80%a7%e6%95%b0%e5%ad%97pcr%e5%ae%9a%e9%87%8f%e6%96%b9%e6%b3%95%e7%a0%94%e7%a9%b6","status":"publish","type":"docs","link":"https:\/\/vibrojet-tech.com\/?docs=%e6%96%b0%e5%9e%8b%e5%86%a0%e7%8a%b6%e7%97%85%e6%af%92b-1-1-7%e5%8f%98%e5%bc%82%e6%a0%aa%e7%89%b9%e5%bc%82%e6%80%a7%e6%95%b0%e5%ad%97pcr%e5%ae%9a%e9%87%8f%e6%96%b9%e6%b3%95%e7%a0%94%e7%a9%b6","title":{"rendered":"\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2 B.1.1.7 \u53d8\u5f02\u682a\u7279\u5f02\u6027\u6570\u5b57 PCR \u5b9a\u91cf\u65b9\u6cd5\u7814\u7a76"},"content":{"rendered":"\n
\n
\n

<\/a>\u300a\u8ba1\u91cf\u5b66\u62a5\u300b<\/p>\n\n\n\n

2023\u5e74\u7b2c5\u671f<\/p>\n<\/div>\n\n\n\n

\n

DOI:<\/p>\n\n\n\n

<\/a><\/a>10.3969\/j.issn.1000-1158.2023.05.23<\/p>\n<\/div>\n\n\n\n

\u738b\u971e\u00a01<\/sup>\uff0c\u90ed\u82e5\u6656\u00a02<\/sup>\uff0c\u8463\u83b2\u534e 3<\/sup><\/p>\n\n\n\n

\n\n\n\n

Abstract<\/h2>\n\n\n\n

\u7531\u4e8e\u4e16\u754c\u536b\u751f\u7ec4\u7ec7\uff08WHO\uff09\u5c06 B.1.1.7\uff08Alpha\uff09\u5217\u4e3a \u201c\u5173\u5207\u7684\u53d8\u5f02\u682a\u201d\uff0c\u56e0\u6b64\u5efa\u7acb\u9488\u5bf9\u8be5\u6bd2\u682a\u7684\u7279\u5f02\u6027\u9ad8\u7075\u654f\u6570\u5b57 PCR \u68c0\u6d4b\u65b9\u6cd5\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002\u672c\u7814\u7a76\u9996\u5148\u4f9d\u636e\u8be5\u53d8\u5f02\u682a\u523a\u7a81\u86cb\u767d\u4e0aH69\/V70 \u7f3a\u5931\u3001P681H\u3001A570D\u5171 3 \u4e2a\u7279\u5f02\u6027\u7a81\u53d8\u4f4d\u70b9\u8bbe\u8ba1\u5f15\u7269\u4e0e\u63a2\u9488\uff0c\u660e\u786e 3 \u79cd\u68c0\u6d4b\u4f53\u7cfb\u7684\u7ebf\u6027\u8303\u56f4\u4e0e\u5b9a\u91cf\u9650\uff1b\u518d\u9009\u53d6\u5176\u4f59 4 \u79cd \u201c\u5173\u5207\u7684\u53d8\u5f02\u682a\u201d \u5b8c\u6210\u7279\u5f02\u6027\u9a8c\u8bc1\uff0c\u5e76\u5bf9\u6bd4\u4e0d\u540c\u6570\u5b57 PCR \u5e73\u53f0\u53ca\u4e0d\u540c\u68c0\u6d4b\u65b9\u6cd5\u7684\u5b9a\u91cf\u51c6\u786e\u5ea6\u3002\u7ed3\u679c\u8bc1\u5b9e\uff0c\u672c\u7814\u7a76\u5efa\u7acb\u7684\u6570\u5b57 PCR \u65b9\u6cd5\u51c6\u786e\u53ef\u9760\uff0c\u53ef\u5e94\u7528\u4e8e B.1.1.7 \u53d8\u5f02\u682a\u7684\u7cbe\u51c6\u5b9a\u91cf\u68c0\u6d4b\u3002<\/p>\n\n\n\n

Due to B.1.1.7 (Alpha) being listed as a \u201cvariant of concern\u201d by the World Health Organization (WHO), it is particularly important to establish a specific and highly sensitive digital PCR method for it. First, this method required designing primers and probes based on the H69\/V70 deletion, P681H, and A570D specific mutation sites of the spike protein, and determining the linear range and limit of quantitation for the three methods. Subsequently, the specificity of the method was verified using four other variants of concern, and quantitative accuracy was compared using different digital PCR platforms and detection methods. Finally, the results indicated that the established digital PCR method was accurate and reliable, and can be used for quantitative detection of B.1.1.7 variant strains.<\/p>\n\n\n\n

\n

Tag<\/strong>s:<\/h2>\n\n\n\n

\u8ba1\u91cf\u5b66\uff08metrology\uff09\uff1b\u65b0\u578b\u51a0\u72b6\u75c5\u6bd2\uff08SARS-CoV-2\uff09\uff1bB.1.1.7\u53d8\u5f02\u682a\uff08B.1.1.7 variant\uff09\uff1b\u5b9a\u91cf\u68c0\u6d4b\uff08quantitative detection\uff09\uff1b \u6570\u5b57PCR\uff08digital PCR\uff09<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Develops a variant-specific dPCR quantification method for SARS-CoV-2 B.1.1.7, supporting specific viral variant identification and quantification.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"footnotes":""},"doc_category":[30],"doc_tag":[],"class_list":["post-13846","docs","type-docs","status-publish","hentry","doc_category-platform-analytical-performance"],"year_month":"2026-05","word_count":153,"total_views":0,"reactions":{"happy":0,"normal":0,"sad":0},"author_info":{"name":"root","author_nicename":"root","author_url":"https:\/\/vibrojet-tech.com\/?author=1"},"doc_category_info":[{"term_name":"Platform Analytical Performance","term_url":"https:\/\/vibrojet-tech.com\/?doc_category=platform-analytical-performance"}],"doc_tag_info":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13846","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs"}],"about":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/types\/docs"}],"author":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13846"}],"version-history":[{"count":12,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13846\/revisions"}],"predecessor-version":[{"id":14761,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13846\/revisions\/14761"}],"wp:attachment":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13846"}],"wp:term":[{"taxonomy":"doc_category","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_category&post=13846"},{"taxonomy":"doc_tag","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_tag&post=13846"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}