{"id":13844,"date":"2026-05-13T14:49:09","date_gmt":"2026-05-13T06:49:09","guid":{"rendered":"https:\/\/vibrojet-tech.com\/?post_type=docs&p=13844"},"modified":"2026-05-25T15:10:15","modified_gmt":"2026-05-25T07:10:15","password":"","slug":"%e5%9f%ba%e5%9b%a0%e2%85%b6-2%e4%ba%9a%e5%9e%8b%e6%96%b0%e5%9f%8e%e7%96%ab%e7%97%85%e6%af%92%e5%8f%8d%e8%bd%ac%e5%bd%95%e6%95%b0%e5%ad%97pcr%e6%a3%80%e6%b5%8b%e6%96%b9%e6%b3%95%e7%9a%84%e5%bb%ba","status":"publish","type":"docs","link":"https:\/\/vibrojet-tech.com\/?docs=%e5%9f%ba%e5%9b%a0%e2%85%b6-2%e4%ba%9a%e5%9e%8b%e6%96%b0%e5%9f%8e%e7%96%ab%e7%97%85%e6%af%92%e5%8f%8d%e8%bd%ac%e5%bd%95%e6%95%b0%e5%ad%97pcr%e6%a3%80%e6%b5%8b%e6%96%b9%e6%b3%95%e7%9a%84%e5%bb%ba","title":{"rendered":"\u57fa\u56e0 \u2166.2 \u4e9a\u578b\u65b0\u57ce\u75ab\u75c5\u6bd2\u53cd\u8f6c\u5f55\u6570\u5b57 PCR \u68c0\u6d4b\u65b9\u6cd5\u7684\u5efa\u7acb\u4e0e\u5e94\u7528"},"content":{"rendered":"\n
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<\/a>\u300a\u4e2d\u56fd\u52a8\u7269\u68c0\u75ab\u300b<\/p>\n\n\n\n

2023\u5e74\u7b2c5\u671f<\/p>\n<\/div>\n\n\n\n

\n

DOI:<\/p>\n\n\n\n

<\/a><\/a>10.3969\/j.issn.1005-944X.2023.05.016<\/p>\n<\/div>\n\n\n\n

\u738b\u9759\u9759\u00a01<\/sup>\uff0c\u514b\u519b\u5b8f\u00a02<\/sup>\uff0c\u4e8e\u6653\u6167\u00a01<\/sup>\uff0c\u5f6d\u771f\u5947\u00a03<\/sup>\uff0c\u90a2\u5b89\u742a\u00a04<\/sup>\uff0c\u5218\u534e\u96f7\u00a01<\/sup><\/p>\n\n\n\n

\n\n\n\n

Abstract<\/h2>\n\n\n\n

\u57fa\u56e0 \u2166 \u578b\u65b0\u57ce\u75ab\u75c5\u6bd2\uff08NDV\uff09\u662f\u4e25\u91cd\u5371\u5bb3\u6211\u56fd\u5bb6\u79bd\u517b\u6b96\u4e1a\u5065\u5eb7\u53d1\u5c55\u7684\u4e3b\u8981\u75c5\u539f\u4e4b\u4e00\u3002\u56fd\u5bb6\u65b0\u57ce\u75ab\u53c2\u8003\u5b9e\u9a8c\u5ba4\u76d1\u6d4b\u8868\u660e\uff0c\u76ee\u524d\u56fd\u5185 NDV \u6d41\u884c\u6bd2\u682a\u5df2\u7531\u57fa\u56e0 \u2166.1.1 \u4e9a\u578b\u8f6c\u53d8\u4e3a \u2166.2 \u4e9a\u578b\u3002\u4e3a\u5b9e\u73b0\u57fa\u56e0 \u2166.2 \u4e9a\u578b NDV \u7684\u5feb\u901f\u68c0\u6d4b\u548c\u7cbe\u51c6\u5b9a\u91cf\uff0c\u9488\u5bf9\u5176\u56fd\u5185\u6d41\u884c\u6bd2\u682aF<\/em>\u57fa\u56e0\u4fdd\u5b88\u533a\u57df\u8bbe\u8ba1\u4e86\u7279\u5f02\u6027\u5f15\u7269\u548c\u63a2\u9488\uff0c\u5efa\u7acb\u4e86\u4e00\u79cd\u53ef\u5feb\u901f\u68c0\u6d4b\u57fa\u56e0 \u2166.2 \u4e9a\u578b NDV \u7684\u53cd\u8f6c\u5f55\u6570\u5b57 PCR\uff08RT-dPCR\uff09\u65b9\u6cd5\uff0c\u5e76\u8bc4\u4f30\u4e86\u8be5\u65b9\u6cd5\u7684\u654f\u611f\u6027\u3001\u7279\u5f02\u6027\u548c\u91cd\u590d\u6027\u3002\u7ed3\u679c\u663e\u793a\uff1a\u5efa\u7acb\u7684 RT-dPCR \u65b9\u6cd5\u68c0\u6d4b\u4e0b\u9650\u4e3a1.30 copies\/\u03bcL\uff0c\u654f\u611f\u6027\u8f83\u9ad8\uff1b\u7279\u5f02\u6027\u5f3a\uff0c\u4e0e\u57fa\u56e0 \u2165 \u578b\u3001\u216b \u578b NDV \u4ee5\u53ca\u5176\u4ed6\u5e38\u89c1\u79bd\u75c5\u75c5\u6bd2\uff08\u79bd\u6d41\u611f\u75c5\u6bd2\u3001\u9e21\u4f20\u67d3\u6027\u652f\u6c14\u7ba1\u708e\u75c5\u6bd2\u3001\u4f20\u67d3\u6027\u5589\u6c14\u7ba1\u708e\u75c5\u6bd2\u548c\u79bd\u817a\u75c5\u6bd2\uff09\u65e0\u4ea4\u53c9\u53cd\u5e94\uff1b\u91cd\u590d\u6027\u8bd5\u9a8c\u53d8\u5f02\u7cfb\u6570\u4e3a2.6 %\uff0c\u5c0f\u4e8e3 %\uff0c\u91cd\u590d\u6027\u597d\u3002\u5229\u7528\u672c\u65b9\u6cd5\u5bf9\u4ece\u6d3b\u79bd\u5e02\u573a\u91c7\u96c6\u7684 200 \u4efd\u9e21\u53e3\u54bd \/ \u6cc4\u6b96\u8154\u62ed\u5b50\u6837\u54c1\u8fdb\u884c\u68c0\u6d4b\uff0c\u53d1\u73b0\u68c0\u6d4b\u7ed3\u679c\u4e0e\u75c5\u6bd2\u5206\u79bb\u9274\u5b9a\u7ed3\u679c\u4e00\u81f4\u3002\u7ed3\u679c\u8868\u660e\uff0c\u672c\u7814\u7a76\u5efa\u7acb\u7684 RT-dPCR \u65b9\u6cd5\u5177\u6709\u826f\u597d\u7684\u654f\u611f\u6027\u3001\u7279\u5f02\u6027\u3001\u91cd\u590d\u6027\u548c\u51c6\u786e\u6027\uff0c\u53ef\u7528\u4e8e\u57fa\u56e0 \u2166.2 \u4e9a\u578b NDV \u7684\u5feb\u901f\u68c0\u6d4b\u548c\u7cbe\u51c6\u5b9a\u91cf\uff0c\u540c\u65f6\u4e5f\u4e3a\u5176\u6838\u9178\u6807\u51c6\u7269\u8d28\u7684\u7814\u5236\u5960\u5b9a\u4e86\u57fa\u7840\u3002<\/p>\n\n\n\n

Genotype \u2166 Newcastle disease virus (NDV) is one of major pathogens seriously endangering poultry industry in China. Monitoring conducted by National Newcastle Disease Reference Laboratory revealed that current NDV strains have evolved from subtype \u2166.1.1 to \u2166.2. In order to rapidly detect and accurately quantify \u2166.2 subtype NDV, based on the conserved region of F<\/em> gene of its domestic strains, specific primers and probes were designed to establish a reverse transcription digital PCR (RT-dPCR), followed by evaluation on its sensitivity, specificity and repeatability. The results showed that the established method, with high sensitivity, could detect at a low limit of 1.30 copies\/\u03bcL; with strong specificity, failed to crossly react with genotypes \u2165, \u2166 and NDV and other common avian disease viruses (including avian influenza virus, avian infectious bronchitis virus, infectious laryngotracheitis virus and avian adenovirus); and with good repeatability, the coefficient of variation (CV) of repeatability test was 2.6 %, less than 3 %. 200 oropharyngeal\/cloacal swab samples collected from live poultry markets were tested by the method, from which, the results were consistent with those of virus isolation and identification. In conclusion, the method was with high specificity, sensitivity and repeatability, and could be used for rapid detection and accurate quantification of sub-genotype VII.2 NDVs, and would lay a foundation for future development of related reference materials.<\/p>\n\n\n\n

\n

Tag<\/strong>s:<\/h2>\n\n\n\n

\u65b0\u57ce\u75ab\u75c5\u6bd2\uff08NDV\uff09\uff1b\u57fa\u56e0\u2166.2\u4e9a\u578b\uff08sub-genotype\u2166.2\uff09\uff1b\u53cd\u8f6c\u5f55\u6570\u5b57PCR\uff08RT-dPCR\uff09\uff1b\u6027\u80fd\u8bc4\u4f30\uff08performance evaluation\uff09<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Establishes an RT-dPCR method for genotype VII.2 Newcastle disease virus, supporting sensitive identification and quantification of animal pathogen subtypes.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"footnotes":""},"doc_category":[30],"doc_tag":[],"class_list":["post-13844","docs","type-docs","status-publish","hentry","doc_category-platform-analytical-performance"],"year_month":"2026-05","word_count":234,"total_views":0,"reactions":{"happy":0,"normal":0,"sad":0},"author_info":{"name":"root","author_nicename":"root","author_url":"https:\/\/vibrojet-tech.com\/?author=1"},"doc_category_info":[{"term_name":"Platform Analytical Performance","term_url":"https:\/\/vibrojet-tech.com\/?doc_category=platform-analytical-performance"}],"doc_tag_info":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13844","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs"}],"about":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/types\/docs"}],"author":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13844"}],"version-history":[{"count":16,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13844\/revisions"}],"predecessor-version":[{"id":14759,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/13844\/revisions\/14759"}],"wp:attachment":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13844"}],"wp:term":[{"taxonomy":"doc_category","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_category&post=13844"},{"taxonomy":"doc_tag","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_tag&post=13844"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}