{"id":11327,"date":"2025-08-25T17:55:25","date_gmt":"2025-08-25T09:55:25","guid":{"rendered":"http:\/\/vibrojet-tech.com\/?post_type=docs&p=11327"},"modified":"2026-05-26T09:33:45","modified_gmt":"2026-05-26T01:33:45","password":"","slug":"%e6%95%b0%e5%ad%97pcr%e4%b8%8e%e5%ae%9e%e6%97%b6%e8%8d%a7%e5%85%89%e5%ae%9a%e9%87%8fpcr%e6%a3%80%e6%b5%8b%e6%85%a2%e6%80%a7%e9%ab%93%e6%80%a7%e7%99%bd%e8%a1%80%e7%97%85%e6%82%a3%e8%80%85bcr__abl-mrna","status":"publish","type":"docs","link":"https:\/\/vibrojet-tech.com\/?docs=%e6%95%b0%e5%ad%97pcr%e4%b8%8e%e5%ae%9e%e6%97%b6%e8%8d%a7%e5%85%89%e5%ae%9a%e9%87%8fpcr%e6%a3%80%e6%b5%8b%e6%85%a2%e6%80%a7%e9%ab%93%e6%80%a7%e7%99%bd%e8%a1%80%e7%97%85%e6%82%a3%e8%80%85bcr__abl-mrna","title":{"rendered":"\u6570\u5b57 PCR \u4e0e\u5b9e\u65f6\u8367\u5149\u5b9a\u91cf PCR \u68c0\u6d4b\u6162\u6027\u9ad3\u6027\u767d\u8840\u75c5\u60a3\u8005 BCR::ABL mRNA \u6c34\u5e73\u7684\u6bd4\u8f83\u6027\u7814\u7a76"},"content":{"rendered":"\n
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\u300a\u4e2d\u534e\u8840\u6db2\u5b66\u6742\u5fd7\u300b<\/p>\n\n\n\n

2023\u5e74\u7b2c11\u671f<\/p>\n<\/div>\n\n\n\n

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DOI:<\/p>\n\n\n\n

10.3760\/cma.j.issn.0253-2727.2023.11.004<\/p>\n<\/div>\n\n\n\n

\u9ad8\u6c49\u6797\uff0c\u90dd\u73a5\uff0c\u9648\u6587\u654f\uff0c\u674e\u73b2\u5a23\uff0c\u738b\u65ed\uff0c\u79e6\u4e9a\u6eb1\uff0c\u6c5f\u5029<\/p>\n\n\n\n

\n\n\n\n

Abstract<\/h2>\n\n\n\n
\n
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\u76ee\u7684\uff1a<\/p>\n\n\n\n

\u6bd4\u8f83\u6570\u5b57 PCR\uff08dPCR\uff09\u4e0e\u5b9e\u65f6\u8367\u5149\u5b9a\u91cf PCR\uff08qPCR\uff09\u65b9\u6cd5\u68c0\u6d4b\u6162\u6027\u9ad3\u6027\u767d\u8840\u75c5\uff08CML\uff09\u60a3\u8005\u5916\u5468\u8840 BCR::ABL\uff08P210\uff09 mRNA \u6c34\u5e73\u7684\u5dee\u5f02\u6027\u3001\u76f8\u5173\u6027\u4e0e\u4e00\u81f4\u6027\u3002<\/p>\n<\/div>\n\n\n\n

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\u65b9\u6cd5\uff1a<\/strong><\/p>\n\n\n\n

\u672c\u7814\u7a76\u4e3a\u975e\u5e72\u9884\u6027\u3001\u6a2a\u65ad\u9762\u7814\u7a76\u3002\u6536\u96c6 2021 \u5e74 9 \u6708\u81f3 2023 \u5e74 2 \u6708\u5728\u5317\u4eac\u5927\u5b66\u4eba\u6c11\u533b\u9662\u5c31\u8bca\u7684\u670d\u7528\u916a\u6c28\u9178\u6fc0\u9176\u6291\u5236\u5242\uff08TKI\uff09\u81f3\u5c11\u83b7\u5f97\u5b8c\u5168\u7ec6\u80de\u9057\u4f20\u5b66\u53cd\u5e94\uff08CCyR\uff09\u7684 CML \u60a3\u8005\u540c\u65f6\u91c7\u7528 dPCR \u548c qPCR \u65b9\u6cd5\u68c0\u6d4b BCR::ABL mRNA \u6c34\u5e73\u7684\u7ed3\u679c\uff0c\u5206\u522b\u91c7\u7528 Wilcoxon \u7b26\u53f7\u79e9\u68c0\u9a8c\u3001Spearman \u76f8\u5173\u7cfb\u6570\u3001Bland-Altman \u5206\u6790\u6bd4\u8f83\u4e24\u79cd\u65b9\u6cd5\u7684\u5dee\u5f02\u6027\u3001\u76f8\u5173\u6027\u3001\u4e00\u81f4\u6027\u3002<\/p>\n<\/div>\n\n\n\n

\n

\u7ed3\u679c\uff1a<\/strong><\/p>\n\n\n\n

356 \u4f8b CML \u60a3\u8005\u7684 459 \u5bf9\u5916\u5468\u8840\u6837\u672c\uff0c\u5206\u522b\u91c7\u7528 dPCR \u4e0e qPCR \u65b9\u6cd5\u68c0\u6d4b BCR::ABL mRNA \u6c34\u5e73\u5e76\u7eb3\u5165\u7edf\u8ba1\u5206\u6790\u3002\u6574\u4f53\u800c\u8a00\uff0c\u4e24\u79cd\u65b9\u6cd5\u68c0\u6d4b\u7ed3\u679c\u5dee\u5f02\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\uff08P<\/em> < 0.001\uff09\u3002\u6309\u5206\u5b50\u5b66\u53cd\u5e94\u5206\u5c42\u5206\u6790\u663e\u793a\uff0c\u8be5\u5dee\u5f02\u4ec5\u5b58\u5728\u4e8e\u8fbe\u5230 MR4.5 \u53ca\u4ee5\u4e0a\u6c34\u5e73\u8005\uff08P<\/em> < 0.001\uff09\uff1b\u672a\u8fbe\u4e3b\u8981\u5206\u5b50\u5b66\u53cd\u5e94\uff08MMR\uff09\uff08P<\/em> = 0.922\uff09\u3001\u8fbe\u5230 MMR\uff08P<\/em> = 0.723\uff09\u53ca\u5206\u5b50\u5b66\u53cd\u5e94 4\uff08MR4\uff09\uff08P<\/em> = 0.099\uff09\u5c42\u7ea7\u5747\u65e0\u660e\u663e\u5dee\u5f02\u3002\u603b\u4f53\u5206\u6790\u53ef\u89c1\uff0cdPCR \u4e0e qPCR \u68c0\u6d4b BCR::ABL mRNA \u6c34\u5e73\u5448\u4e2d\u5ea6\u76f8\u5173\uff08r<\/em> = 0.761\uff0cP<\/em> < 0.001\uff09\u3002\u968f\u5206\u5b50\u5b66\u53cd\u5e94\u7a0b\u5ea6\u52a0\u6df1\uff0c\u4e8c\u8005\u76f8\u5173\u6027\u9010\u6b65\u964d\u4f4e\u76f4\u81f3\u65e0\u660e\u663e\u76f8\u5173\uff1a\u672a\u8fbe MMR\uff08r<\/em> = 0.929\uff0cP<\/em> < 0.001\uff09\u3001MMR\uff08r<\/em> = 0.815\uff0cP<\/em> < 0.001\uff09\u3001MR4\uff08r<\/em> = 0.408\uff0cP<\/em> < 0.001\uff09\u3001MR4.5\uff08r<\/em> = 0.176\uff0cP<\/em> = 0.176\uff09\u3002\u4e00\u81f4\u6027\u5206\u6790\u7ed3\u679c\u63d0\u793a\uff0cMR4.5 \u7ec4\u4e24\u79cd\u68c0\u6d4b\u65b9\u6cd5\u7684\u4e00\u81f4\u6027\u663e\u8457\u5dee\u4e8e\u5176\u4f59\u5206\u5c42\uff1a\u672a\u8fbe MMR\uff08r<\/em> = 0.042\uff0cP<\/em> = 0.846\uff09\u3001MMR\uff08r<\/em> = 0.054\uff0cP<\/em> = 0.229\uff09\u3001MR4\uff08r<\/em> = \u22120.020\uff0cP<\/em> = 0.399\uff09\u3001MR4.5\uff08r<\/em> = \u22120.219\uff0cP<\/em> < 0.001\uff09\u3002<\/p>\n<\/div>\n\n\n\n

\n

\u7ed3\u8bba\uff1a<\/strong><\/p>\n\n\n\n

CML \u60a3\u8005\u5f53\u83b7\u5f97\u6df1\u5c42\u5206\u5b50\u5b66\u53cd\u5e94\u540e\uff0c\u66f4\u9002\u5408\u91c7\u7528 dPCR \u65b9\u6cd5\u76d1\u6d4b BCR::ABL\uff08P210\uff09mRNA \u6c34\u5e73\u4ee5\u63d0\u9ad8\u7cbe\u51c6\u6027\u3002<\/p>\n<\/div>\n\n\n\n

\n

Objective: <\/p>\n\n\n\n

To compare digital polymerase chain reaction (dPCR) and real-time quantitative PCR (qPCR) measurements of BCR::ABL (P210) mRNA expression in patients with chronic myeloid leukemia (CML).<\/p>\n<\/div>\n\n\n\n

\n

Methods: <\/strong><\/p>\n\n\n\n

In this non-interventional, cross-sectional study, BCR::ABL (P210) mRNA was simultaneously measured by dPCR and qPCR in peripheral blood samples collected from patients with CML who underwent tyrosine kinase inhibitor therapy and who achieved at least a complete cytogenetic response from September 2021 to February 2023 at Peking University People’s Hospital. The difference, correlation, and agreement between the two methods were evaluated using the Wilcoxon signed-rank test, Spearman’s correlation, and Bland-Altman analysis, respectively.<\/p>\n<\/div>\n\n\n\n

\n

Results: <\/strong><\/p>\n\n\n\n

In total, 459 data pairs for BCR::ABL mRNA expression measured by dPCR and qPCR from 356 patients with CML were analyzed. There was a significant difference in BCR::ABL mRNA expression between the two methods (P<\/em> < 0.001). When analyzed by the depth of the molecular response (MR), a significant difference only existed for patients with \u2265 MR4.5 (P<\/em> < 0.001). No significant difference was observed for those who did not achieve a major MR (no MMR;\u00a0P<\/em> = 0.922) or for those who achieved a major MR (MMR;\u00a0P<\/em> = 0.723) or MR4 (P<\/em> = 0.099). There was a moderate correlation between the BCR::ABL mRNA expression between the two methods (r<\/em> = 0.761,\u00a0P<\/em> < 0.001). However, the correlation gradually weakened or disappeared as the depth of the MR increased (no MMR:\u00a0r<\/em> = 0.929,\u00a0P<\/em> < 0.001; MMR:\u00a0r<\/em> = 0.815,\u00a0P<\/em> < 0.001; MR4:\u00a0r<\/em> = 0.408,\u00a0P<\/em> < 0.001; MR4.5:\u00a0r<\/em> = 0.176,\u00a0P<\/em> = 0.176). In addition, the agreement in BCR::ABL mRNA expression between the two methods in those with MR4.5 was weaker than other groups (no MMR:\u00a0r<\/em> = 0.042,\u00a0P<\/em> = 0.846; MMR:\u00a0r<\/em> = 0.054,\u00a0P<\/em> = 0.229; MR4:\u00a0r<\/em> = -0.020,\u00a0P<\/em> = 0.399; MR4.5:\u00a0r<\/em> = -0.219,\u00a0P<\/em> < 0.001) .<\/p>\n<\/div>\n\n\n\n

\n

Conclusion: <\/strong><\/p>\n\n\n\n

dPCR is more accurate than qPCR for measuring BCR::ABL (P210) mRNA expression in patients with CML who achieve a stable deep MR.<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n

Tag<\/strong>s:<\/h2>\n\n\n\n

\u767d\u8840\u75c5\uff0c\u9ad3\u6837\uff0c\u6162\u6027\uff08Leukemia, myeloid, chronic\uff09\uff1b \u5b9e\u65f6\u5b9a\u91cf\u805a\u5408\u9176\u94fe\u53cd\u5e94\uff08 Real- time quantitative polymerase chain reaction\uff09\uff1b \u6570\u5b57\u805a\u5408\u9176\u94fe\u53cd\u5e94\uff08Digital polymerase chain reaction\uff09\uff1b \u878d\u5408\u86cb\u767d\u8d28\u7c7b\uff0cBCR::ABL\uff08Fusion proteins, BCR::ABL\uff09<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Compares digital PCR with RT-qPCR for BCR-ABL mRNA measurement in CML patients, supporting clinical value of dPCR in molecular treatment monitoring.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"footnotes":""},"doc_category":[32],"doc_tag":[],"class_list":["post-11327","docs","type-docs","status-publish","hentry","doc_category-clinical-translational-evidence"],"year_month":"2026-05","word_count":407,"total_views":0,"reactions":{"happy":0,"normal":0,"sad":0},"author_info":{"name":"root","author_nicename":"root","author_url":"https:\/\/vibrojet-tech.com\/?author=1"},"doc_category_info":[{"term_name":"Clinical & Translational Evidence","term_url":"https:\/\/vibrojet-tech.com\/?doc_category=clinical-translational-evidence"}],"doc_tag_info":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/11327","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs"}],"about":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/types\/docs"}],"author":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11327"}],"version-history":[{"count":5,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/11327\/revisions"}],"predecessor-version":[{"id":14834,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=\/wp\/v2\/docs\/11327\/revisions\/14834"}],"wp:attachment":[{"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11327"}],"wp:term":[{"taxonomy":"doc_category","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_category&post=11327"},{"taxonomy":"doc_tag","embeddable":true,"href":"https:\/\/vibrojet-tech.com\/index.php?rest_route=%2Fwp%2Fv2%2Fdoc_tag&post=11327"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}